beijing1 beijing2
                    客 服 熱 線
                    400-004-3669
                    懸浮細胞免疫熒光專用玻片Shi-Fix CoverSlips
                    懸浮細胞免疫熒光專用玻片
                    SHI-FIX? coverslips
                    • 產品編號: SB-Shifix25

                    • 相關CAS號:

                    • 分子式:

                    • 分子量:

                    訂貨號 規格 價格 庫存 數量 購買
                    SB-Shifix25 25片/盒 1800.00元 10

                    細胞免疫熒光主要用于蛋白定位研究,相互作用研究和細胞信號轉導研究。細胞免疫熒光就是將免疫學方法和熒光標記技術相結合,研究特異性抗原在細胞內的分布。細胞免疫熒光特性高,敏感性強,速度快,主要原理也是利用抗原抗體反映抗原抗體結合后再用熒光標記,通過顯微鏡下觀察來確定某種特異性抗原是否存在。

                    根據培養的細胞類型,可分為貼壁細胞懸浮細胞(淋巴細胞,血液的白細胞)。貼壁細胞有天然貼壁的屬性,而懸浮細胞不依賴支持物表面,在培養液中呈懸浮狀態生長。

                    免疫熒光實驗的基礎前提就是讓細胞能固定在玻片上。懸浮細胞如何貼壁或者固定到玻片上,是全世界科研人員面對的一個難題。傳統上,或用細胞離心甩片機制備細胞片或直接涂片法制備細胞涂片,然后把細胞片于乙醇或丙酮或多聚甲醛固定。無論甩片還是涂片,都是黑盒子實驗。每個人操作手法不一樣,即使同一個人操作,每一批實驗,也都沒法評價細胞片上的細胞固定的情況,而這一步至關重要。盲目而又沒底的只能硬著頭皮往下做。浪費的不僅是時間,還有抗體等很多試劑。如何解決這些痛點?懸浮細胞免疫熒光專用玻片Shi-Fix Coverslips帶來終極解決方案!


                    您是否希望懸浮細胞的免疫熒光像貼壁細胞一樣容易?現在就是這樣!

                    Shi-fix?玻片允許懸浮細胞分層或直接作為單層生長。簡單,無憂的實驗方案,無需離心即可將懸浮細胞固定到載玻片上。只需將細胞添加到載玻片上,等待30分鐘,用PBS洗滌未結合的細胞并繼續免疫染色(或繼續培養以獲得所需的細胞密度)。

                    這些玻片也可用于染色懸浮細胞以進行顯微鏡檢查,或用于固定懸浮細胞以進行共聚焦顯微鏡檢查。更重要的是,如果需要,您還可以在細胞附著在玻片上時刺激它們。您可以像貼壁細胞一樣處理非貼壁細胞!

                    Do you wish suspension cell Immunofluorescence was as easy as that of adherent cells? 

                    Now it is! Our innovative Shi-fix? cover-slips or multi well plates allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining.Or continue the culture to obtain desired cell densities for suspension cell immunofluorescence. 

                    These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so. 

                    coverslips 1.PNG

                    coverslips 2.PNG

                    產品訂購信息:

                    產品訂購信息

                    使用該產品的部分文獻:

                    1. Genetic Disruption of Transfer RNA Modifications in Human Cancer. Laia Coll San Martin. Universitat De Barcelona, Facultat De Medicina Doctoral Thesis 2021

                    2. Inhibition of NETosis by a Nuclear-Penetrating Anti-DNA Autoantibody. Xiaoyong Chen, Benedette J. Cuffari, Valentina Dubljevic, et al. ImmunoHorizons June 1, 2022, 6 (6) 356-365; DOI: https://doi.org/10.4049/immunohorizons.2100091

                    3. Coming in and Finding Out: Blending Receptor-Targeted Delivery and Efficient Endosomal Escape in a Novel Bio-Responsive siRNA Delivery System for Gene Knockdown in Pulmonary T Cells. Kandil R, Xie Y, Heermann R, et al. Adv Ther (Weinh). 2019;2(7):1900047. doi:10.1002/adtp.201900047 

                    4. Specific Targeting of Somatostatin Receptor Subtype-2 for Fluorescence-Guided Surgery Servando Hernandez Vargas et al. Clin Cancer Res July 15 2019 25 (14) 4332-4342; DOI:10.1158/1078-0432.CCR-18-3312

                    5. Carboxy-terminal dendrimers with phenylalanine for a pH-sensitive delivery system into immune cells including T cells. Hiroya Shiba et al. 2022 Apr 6;10(14):2463-2470. doi: 10.1039/d1tb01980e.

                    6. Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Jeffrey R. Whiteaker and Tao Wang et al. Cancers 2021, 13(15), 3843; https://doi.org/10.3390/cancers13153843


                    Shi-Fix說明書:

                    1. auto_1455.pngShi-fix? Coverslips 產品說明書下載

                    2. auto_1455.pngShi-fix? Plates 產品說明書下載

                    SHI-FIX-P-多色

                    1. Wash cells with PBS and resuspend 2-5 million cells per ml in PBS 

                    2. Place the Shi-fix? cover-slips on a 12-well ELISA plate for ease of handling. Add the cells directly to Shi-fix? cover-slips.(0.2-0.3 million cells per coverslip). 

                    3. Let the cells settle and attach for 30 mins. Attachment for some cell types may improve by incubating longer but do not exceed 1 hour. Wash gently with 2ml 

                        PBS to  remove unbound cells (Do not pipet PBS straight onto the coverslips. Add PBS to the sides of the plate well followed by gentle rocking for 3-5 mins). 

                    4. Check for cell attachment on a microscope. If further culturing is needed, add 1ml medium otherwise proceed to fixation, permeabilization and 

                        immunostaining.

                    搜索質檢報告(COA)
                    搜索MSDS
                    成人免费无码成人影院日韩_国产精品VA在线播放我和闺蜜_国产亚洲精品自在久久_国产免费AV片无码永久免费